The Sicilians - currently lying in second place in Serie A, three points behind Inter - travel to face the Turkish side in Istanbul amid claims they are more interested in the showdown at Stadio Renzo Barbera at the weekend.
"It's not true that we are only interested in Serie A," Guidolin said. "Our intention is to try to do well in all three competitions. It is however only normal that I must make some choices, as it's impossible to use the same squad all the time."
Palermo's commitment to their UEFA Cup cause has already been called into question this season, with president Maurizio Zamparini claiming he was "not interested" in the competition ahead of the tie against West Ham.
The islanders lie in second place in Group H, with Newcastle topping the pool. Fenerbahce have one point from just one outing so far.
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